Services centres along the Hume Highway at Pheasants Nest will be closed until next month ahead of redevelopment work.
There will temporarily be no access to both the northbound and southbound service centres between Remembrance Driveway and Wilton Road until Sunday, August 2, weather permitting.
This means motorists will be unable to access the shops and refuelling facilities at either station.
Transport for NSW regional director Jo Parrott said motorists would still be able to utilise toilet facilities.
"Toilet facilities and limited parking will remain open during the temporary closure of these services while the remainder of the site will be fenced off and closed," she said.
The nearest petrol stations for vehicles travelling south are the Shell Service Station at Wilton Road, Wilton (five kilometres north) and the Shell Service Station, Old Hume Highway, Balaclava (23 kilometres south).
The closest rest area for southbound vehicles is just south of the Bargo off ramp at Yanderra.
The nearest petrol stations for motorists heading north are the BP Service Station at Remembrance Driveway, Bargo (seven kilometres south) and the Douglas Park Service Station at Camden Road, Douglas Park (13 kilometres north).
The closest rest area for northbound vehicles on the Hume Highway is at Berrima.
The Shell Highway Services Centre is the nearest dual access facility for both northbound and southbound vehicles and is located 52 kilometres south of the Pheasants Nest Highway Services Centre on the Hume Highway in Sutton Forest.
Ms Parrott said shop and refuelling facilities would reopen from Monday, August 3 until the redevelopment commences.
"The redevelopment work will be staged to minimise impact on customers and the broader community who will be advised of the redevelopment work in advance of the work occurring," she said.
"The new highway service centres on both the north and southbound sides will incorporate a modern, innovative design that offers a range of refuelling and rest facilities that meet varied customer needs."
Work is expected to be completed by early 2022.